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Extraction, identification, high-throughput <t>lncRNA</t> chip <t>sequencing,</t> and differentially expressed lncRNA selection of urinary exosomes isolated from bladder cancer (BCa) patients. (A) Transmission electron microscopy (scale bar = 100 nm). (B) Western blot. (C) Nanoparticle tracking analysis. (D) A total of 35 lncRNAs were significantly up-regulated in the intersection datasets of tumor versus normal tissue and high-stage tumor versus low-stage tumor. (E) The expression level of the top 11 up-regulated lncRNAs was validated in urinary exosomes from BCa patients (i) and BCa cell lines (ii). (F) lnc-TAF12–2:1 was highly expressed in urinary exosomes (i) and tumor tissues of BCa patients (ii), as well as in BCa cell lines (iii).
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Extraction, identification, high-throughput lncRNA chip sequencing, and differentially expressed lncRNA selection of urinary exosomes isolated from bladder cancer (BCa) patients. (A) Transmission electron microscopy (scale bar = 100 nm). (B) Western blot. (C) Nanoparticle tracking analysis. (D) A total of 35 lncRNAs were significantly up-regulated in the intersection datasets of tumor versus normal tissue and high-stage tumor versus low-stage tumor. (E) The expression level of the top 11 up-regulated lncRNAs was validated in urinary exosomes from BCa patients (i) and BCa cell lines (ii). (F) lnc-TAF12–2:1 was highly expressed in urinary exosomes (i) and tumor tissues of BCa patients (ii), as well as in BCa cell lines (iii).

Journal: Genes & Diseases

Article Title: Urinary exosomal lnc-TAF12–2:1 promotes bladder cancer progression through the miR-7847–3p/ASB12 regulatory axis

doi: 10.1016/j.gendis.2024.101384

Figure Lengend Snippet: Extraction, identification, high-throughput lncRNA chip sequencing, and differentially expressed lncRNA selection of urinary exosomes isolated from bladder cancer (BCa) patients. (A) Transmission electron microscopy (scale bar = 100 nm). (B) Western blot. (C) Nanoparticle tracking analysis. (D) A total of 35 lncRNAs were significantly up-regulated in the intersection datasets of tumor versus normal tissue and high-stage tumor versus low-stage tumor. (E) The expression level of the top 11 up-regulated lncRNAs was validated in urinary exosomes from BCa patients (i) and BCa cell lines (ii). (F) lnc-TAF12–2:1 was highly expressed in urinary exosomes (i) and tumor tissues of BCa patients (ii), as well as in BCa cell lines (iii).

Article Snippet: lncRNA chip sequencing of total RNA in urine exosomes was entrusted to Obio Technology (Shanghai, China).

Techniques: Extraction, High Throughput Screening Assay, ChIP-sequencing, Selection, Isolation, Transmission Assay, Electron Microscopy, Western Blot, Expressing